# **Map** >[!quote] >Life is not found in atoms or molecules or genes as such, but in organization; not in symbiosis but in synthesis. >In 'Cell and Protoplasm Concepts: Historical Account', The Cell and the Protoplasm: Publication of the American Association of Science (1940), No. 114, >[Edwin Grant Conklin](https://todayinsci.com/C/Conklin_Edwin/ConklinEdwin-Quotations.html) - [[0040 HDAC, Methylation]] - SWI/SNF Chromatin Remodelling Complex - PRC2 – EZH2 - LSD1 - PVRL2 / CD112? - E2F Family Transcription Network - Physical Structure considerations (gene access etc) - FOXM1 ## Overview Different levels of epigenetics Methylation Promoter regions etc Transcription factors Histone deacetylsation * * HDAC ![[DNA Methylation Graphic Epigenetics.jpg]] — just general overview / intro Multiplex targeting – often difficult or risky to apply with nuclease-based gene-editing tools, is a transformative step for unlocking the scope of cell therapies. Such multiplex epi-editing has the potential to increase therapeutic performance, guiding CAR-T cells towards durable phenotypes that resist cellular exhaustion or that can adapt to the suppressive microenvironments around solid tumours. Epi-editing tools are inherently multiplexable, allowing all manner of complex, polygenic mechanisms to be targeted. This transformative potential could be used to fine-tune the gene expression patterns involved in metabolic reprogramming, adhesion, and migration. These modifications could then enable engineered T cells to penetrate dense tumour stromas and sustain activity in hostile environments, or to guide the differentiation of stem cells towards desired lineages for novel regenerative medicines. https://www.ddw-online.com/unlocking-the-future-of-medicine-with-cell-therapy-32949-202412/ — Does radiation affect epigenetics as part of its effective priming of the TME? Immune epigenetics improvements? Car-t : so much of immune cellular performance is based on epigenetics. Like exhausted T Cells having transitioned to a specific exhausted epigenetic profile. This makes senses adaptively… typical Darwinian evolution and feedback mechanisms to keep immune system in balance and prevent cytotoxic T cells from overrunning and then causing lethal autoimmune issues. Some sort of feedback loop where they run out of juice eventually and develop an exhausted phenotype --- https://www.the-scientist.com/epigenetics-and-heritable-control-of-gene-expression-72425 Read later chromatin packing and chromatin folding comes into play ### LSD 1